Colorectal cancer organizations

colorectal cancer organizations

Purpose: The phytochemical profile and anticancer potential of three Ajuga sp. Materials and Methods: The phytochemicals were extracted from the aerial parts of Ajuga sp.

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The phytochemical profile was also evaluated by principal component analysis in connection with antitumor efficacy of extracts. Western Blot with regard to inflammatory protein NF-κB nuclear factor kappa-light-chain-enhancer of activated B cells p65 subunit expression in cell lysates was performed.

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Enzymatic and non-enzymatic antioxidant capability was assessed by measuring catalase activity and by evaluating the total antioxidant capacity TAC of treated cells.

Results: Ajuga laxmannii ethanol extract showed the highest total phenolic and flavonoid content, while A. The overall cytostatic effect of the investigated plant extracts was exerted through strong inhibitory actions on NF-κB, the key molecule involved in the inflammatory response and via oxidative stress modulatory effects in both murine colon carcinoma and melanoma cell lines.

Conclusion: Ajuga laxmannii showed the most significant antitumor activity and represents an important source of bioactive compounds, possibly an additional form of treatment alongside conventional colorectal cancer organizations drugs.

Ann Ital Chir ; The purpose of our study was to analyse the immediate and remote results obtained after MOR and to identify potential factors that might influence the outcome. We excluded patients with hepatic metastatic tumors and those who needed pelvic exenteration.

Introduction Medicinal plants have always been an important source for various pharmaceuticals since ancient times. Nowadays the scientific interest for new drugs production from bioactive compounds isolated from natural products is still growing.

Herbal medicines were often used only based on empirical observations since antiquity, without knowing the phytochemicals from the extracts or details of their pharmacological effects Atanasov et al. Although many herbal remedies have a well-known composition and certain biological effects, some of them are still used only based on traditional medicine, and lacking the validation of colorectal cancer organizations safety and efficacy.

The research on unexplored medicinal plants traditionally used in folk medicine could determine the development of novel herbal formulations colorectal cancer organizations significant biological activities.

Original Research ARTICLE

Due to their important pharmacological effects, the natural compounds are effectively colorectal cancer organizations to obtain new phytomedicines. Ajuga species Lamiaceaewhich are widely distributed in many parts of the world Atay et al. Six Ajuga species are mentioned in the Romanian spontaneous flora, with Ajuga genevensis L.

Our previous research showed the antioxidant, antimicrobial, and anti-inflammatory colorectal cancer organizations of aerial parts extracts Toiu et al. The monoterpene glycosides content and the essential oil composition have been recently studied on species from Italy, together with the evaluation of antioxidant activity and cytotoxicity by MTT assay Venditti et al.

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The colorectal cancer organizations activity of natural compounds is attributed to their synergistically acting complex mixture of phytochemicals with chemopreventive and chemotherapeutic potential, which can prove to be far more effective than isolated bioactive molecules de Kok et al.

Accordingly, the unexplored plants used in folk medicine require extensive studies for reliable evidence-based phytotherapy. Although complementary and alternative ethnopharmacological approaches are mainly focused on counteracting the side effects and collateral symptoms of conventional cancer therapies, in this paper we investigated a potential disjunction change in traditional plant use Leonti and Casu,by assessing the anticancer activity of these indigenous herbs.

Therefore, this colorectal cancer organizations was aimed to perform a comparative phytochemical analysis of A. F10 murine melanoma and C26 colon carcinoma cells.

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Both cell lines are characterized by increased metastatic potential and are prone to therapeutic alterations of their redox status Rauca et al. In addition, melanoma and colon carcinoma are two of the deadliest cancers in modern society, possibly interlinked by epigenetic mechanisms, as recent reports concluded that colorectal cancer is one of the most common discordant cancers post-melanoma Frank et al.

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As previously reported, the highly metastatic B The colorectal cancer organizations synergistic interaction between bioactive constituents suggests that the whole plant extract may contribute to better therapeutic outcomes compared to the administration of single isolated compounds at an equivalent dose Rasoanaivo et al.

Materials and Methods Chemicals and Reagents High purity chemicals: sodium carbonate, sodium acetate trihydrate, and anhydrous aluminum chloride were acquired from Sigma-Aldrich Germany. Folin-Ciocâlteu reagent was purchased from Merck Germany. HPLC grade solvents methanol, acetonitrile, ammonium acetate, and silver nitrate were purchased from Sigma-Aldrich.

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Preparation of Standard Solutions Standard stock solutions of the flavonoids and iridoids were prepared by dissolving 1 mg of each compound in 1 mL methanol and stored at 4°C, protected from daylight. They were appropriately diluted with double distilled water foot wart diagram being used as working solutions.

Plant Samples and Extraction Procedures The aerial parts of Ajuga species collected in flowering stage in June were obtained and authenticated by one of us A.

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The dried plant samples were ground colorectal cancer organizations a fine powder before extraction. The aerial parts extracts of A.

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The liquid chromatograph was equipped with binary gradient pump, degasser, column thermostat and autosampler. The chromatographic separation was colorectal cancer organizations on a reversed-phase Zorbax SB-C18 mm × 3.

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The column temperature was set at 48°C. The MS system operated using an electrospray ion source in negative mode. The identification and quantification of polyphenols were made in UV assisted by Colorectal cancer organizations. Quantitative determinations were performed using an external standard method.

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In order to determine the concentration of polyphenols in plant samples, colorectal cancer organizations calibration curves in the range of 0. The compounds were identified by comparison of their retention times and the recorded ESI-MS with those of standards in the same chromatographic conditions Vlase et al.

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The LC was equipped with colorectal cancer organizations binary pump, autosampler, thermostat and detector all Series from Agilent Inc. The system was controlled with Data Analysis software version B

  1. Although the etiology is not yet completely known, colorectal cancer is considered a disease with multiple factors, an important place occupying the impact of the environmental factors on the genetically particularly predisposition of patients.
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